what should support a hypothesis and conclusion in an experiment

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How to Write a Good Lab Conclusion in Science

Last Updated: March 21, 2024 Fact Checked

This article was co-authored by Bess Ruff, MA . Bess Ruff is a Geography PhD student at Florida State University. She received her MA in Environmental Science and Management from the University of California, Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the Caribbean and provided research support as a graduate fellow for the Sustainable Fisheries Group. There are 11 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 1,762,905 times.

A lab report describes an entire experiment from start to finish, outlining the procedures, reporting results, and analyzing data. The report is used to demonstrate what has been learned, and it will provide a way for other people to see your process for the experiment and understand how you arrived at your conclusions. The conclusion is an integral part of the report; this is the section that reiterates the experiment’s main findings and gives the reader an overview of the lab trial. Writing a solid conclusion to your lab report will demonstrate that you’ve effectively learned the objectives of your assignment.

Outlining Your Conclusion

• Restate : Restate the lab experiment by describing the assignment.
• Explain : Explain the purpose of the lab experiment. What were you trying to figure out or discover? Talk briefly about the procedure you followed to complete the lab.
• Results : Explain your results. Confirm whether or not your hypothesis was supported by the results.
• Uncertainties : Account for uncertainties and errors. Explain, for example, if there were other circumstances beyond your control that might have impacted the experiment’s results.
• New : Discuss new questions or discoveries that emerged from the experiment.

• Your assignment may also have specific questions that need to be answered. Make sure you answer these fully and coherently in your conclusion.

Discussing the Experiment and Hypothesis

• If you tried the experiment more than once, describe the reasons for doing so. Discuss changes that you made in your procedures.
• Brainstorm ways to explain your results in more depth. Go back through your lab notes, paying particular attention to the results you observed. [5] X Trustworthy Source University of North Carolina Writing Center UNC's on-campus and online instructional service that provides assistance to students, faculty, and others during the writing process Go to source

• Start this section with wording such as, “The results showed that…”
• You don’t need to give the raw data here. Just summarize the main points, calculate averages, or give a range of data to give an overall picture to the reader.
• Make sure to explain whether or not any statistical analyses were significant, and to what degree, such as 1%, 5%, or 10%.

• Use simple language such as, “The results supported the hypothesis,” or “The results did not support the hypothesis.”

Demonstrating What You Have Learned

• If it’s not clear in your conclusion what you learned from the lab, start off by writing, “In this lab, I learned…” This will give the reader a heads up that you will be describing exactly what you learned.
• Add details about what you learned and how you learned it. Adding dimension to your learning outcomes will convince your reader that you did, in fact, learn from the lab. Give specifics about how you learned that molecules will act in a particular environment, for example.
• Describe how what you learned in the lab could be applied to a future experiment.

• On a new line, write the question in italics. On the next line, write the answer to the question in regular text.

• If your experiment did not achieve the objectives, explain or speculate why not.

Wrapping Up Your Conclusion

• If your experiment raised questions that your collected data can’t answer, discuss this here.

• Describe what is new or innovative about your research.
• This can often set you apart from your classmates, many of whom will just write up the barest of discussion and conclusion.

Finalizing Your Lab Report

Community Q&A

• If you include figures or tables in your conclusion, be sure to include a brief caption or label so that the reader knows what the figures refer to. Also, discuss the figures briefly in the text of your report. Thanks Helpful 0 Not Helpful 0
• Once again, avoid using personal pronouns (I, myself, we, our group) in a lab report. The first-person point-of-view is often seen as subjective, whereas science is based on objectivity. Thanks Helpful 0 Not Helpful 0
• Ensure the language used is straightforward with specific details. Try not to drift off topic. Thanks Helpful 0 Not Helpful 0

• Take care with writing your lab report when working in a team setting. While the lab experiment may be a collaborative effort, your lab report is your own work. If you copy sections from someone else’s report, this will be considered plagiarism. Thanks Helpful 3 Not Helpful 0

You Might Also Like

• ↑ https://phoenixcollege.libguides.com/LabReportWriting/introduction
• ↑ https://www.hcs-k12.org/userfiles/354/Classes/18203/conclusionwriting.pdf
• ↑ https://www.education.vic.gov.au/school/teachers/teachingresources/discipline/english/literacy/Pages/puttingittogether.aspx
• ↑ https://writingcenter.unc.edu/tips-and-tools/brainstorming/
• ↑ https://advice.writing.utoronto.ca/types-of-writing/lab-report/
• ↑ http://www.socialresearchmethods.net/kb/hypothes.php
• ↑ https://libguides.usc.edu/writingguide/conclusion
• ↑ https://libguides.usc.edu/writingguide/introduction/researchproblem
• ↑ http://writingcenter.unc.edu/handouts/scientific-reports/
• ↑ https://phoenixcollege.libguides.com/LabReportWriting/labreportstyle
• ↑ https://writingcenter.unc.edu/tips-and-tools/editing-and-proofreading/

About This Article

To write a good lab conclusion in science, start with restating the lab experiment by describing the assignment. Next, explain what you were trying to discover or figure out by doing the experiment. Then, list your results and explain how they confirmed or did not confirm your hypothesis. Additionally, include any uncertainties, such as circumstances beyond your control that may have impacted the results. Finally, discuss any new questions or discoveries that emerged from the experiment. For more advice, including how to wrap up your lab report with a final statement, keep reading. Did this summary help you? Yes No

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The Scientific Method Tutorial

The scientific method, steps in the scientific method.

There is a great deal of variation in the specific techniques scientists use explore the natural world. However, the following steps characterize the majority of scientific investigations:

Step 1: Make observations Step 2: Propose a hypothesis to explain observations Step 3: Test the hypothesis with further observations or experiments Step 4: Analyze data Step 5: State conclusions about hypothesis based on data analysis

Each of these steps is explained briefly below, and in more detail later in this section.

Step 1: Make observations

A scientific inquiry typically starts with observations. Often, simple observations will trigger a question in the researcher's mind.

Example: A biologist frequently sees monarch caterpillars feeding on milkweed plants, but rarely sees them feeding on other types of plants. She wonders if it is because the caterpillars prefer milkweed over other food choices.

Step 2: Propose a hypothesis

The researcher develops a hypothesis (singular) or hypotheses (plural) to explain these observations. A hypothesis is a tentative explanation of a phenomenon or observation(s) that can be supported or falsified by further observations or experimentation.

Example: The researcher hypothesizes that monarch caterpillars prefer to feed on milkweed compared to other common plants. (Notice how the hypothesis is a statement, not a question as in step 1.)

Step 3: Test the hypothesis

The researcher makes further observations and/or may design an experiment to test the hypothesis. An experiment is a controlled situation created by a researcher to test the validity of a hypothesis. Whether further observations or an experiment is used to test the hypothesis will depend on the nature of the question and the practicality of manipulating the factors involved.

Example: The researcher sets up an experiment in the lab in which a number of monarch caterpillars are given a choice between milkweed and a number of other common plants to feed on.

Step 4: Analyze data

The researcher summarizes and analyzes the information, or data, generated by these further observations or experiments.

Example: In her experiment, milkweed was chosen by caterpillars 9 times out of 10 over all other plant selections.

Step 5: State conclusions

The researcher interprets the results of experiments or observations and forms conclusions about the meaning of these results. These conclusions are generally expressed as probability statements about their hypothesis.

Example: She concludes that when given a choice, 90 percent of monarch caterpillars prefer to feed on milkweed over other common plants.

Often, the results of one scientific study will raise questions that may be addressed in subsequent research. For example, the above study might lead the researcher to wonder why monarchs seem to prefer to feed on milkweed, and she may plan additional experiments to explore this question. For example, perhaps the milkweed has higher nutritional value than other available plants.

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The Scientific Method Flowchart

The steps in the scientific method are presented visually in the following flow chart. The question raised or the results obtained at each step directly determine how the next step will proceed. Following the flow of the arrows, pass the cursor over each blue box. An explanation and example of each step will appear. As you read the example given at each step, see if you can predict what the next step will be.

Activity: Apply the Scientific Method to Everyday Life Use the steps of the scientific method described above to solve a problem in real life. Suppose you come home one evening and flick the light switch only to find that the light doesn’t turn on. What is your hypothesis? How will you test that hypothesis? Based on the result of this test, what are your conclusions? Follow your instructor's directions for submitting your response.

The above flowchart illustrates the logical sequence of conclusions and decisions in a typical scientific study. There are some important points to note about this process:

1. The steps are clearly linked.

The steps in this process are clearly linked. The hypothesis, formed as a potential explanation for the initial observations, becomes the focus of the study. The hypothesis will determine what further observations are needed or what type of experiment should be done to test its validity. The conclusions of the experiment or further observations will either be in agreement with or will contradict the hypothesis. If the results are in agreement with the hypothesis, this does not prove that the hypothesis is true! In scientific terms, it "lends support" to the hypothesis, which will be tested again and again under a variety of circumstances before researchers accept it as a fairly reliable description of reality.

2. The same steps are not followed in all types of research.

The steps described above present a generalized method followed in a many scientific investigations. These steps are not carved in stone. The question the researcher wishes to answer will influence the steps in the method and how they will be carried out. For example, astronomers do not perform many experiments as defined here. They tend to rely on observations to test theories. Biologists and chemists have the ability to change conditions in a test tube and then observe whether the outcome supports or invalidates their starting hypothesis, while astronomers are not able to change the path of Jupiter around the Sun and observe the outcome!

3. Collected observations may lead to the development of theories.

When a large number of observations and/or experimental results have been compiled, and all are consistent with a generalized description of how some element of nature operates, this description is called a theory. Theories are much broader than hypotheses and are supported by a wide range of evidence. Theories are important scientific tools. They provide a context for interpretation of new observations and also suggest experiments to test their own validity. Theories are discussed in more detail in another section.

The Scientific Method in Detail

In the sections that follow, each step in the scientific method is described in more detail.

Step 1: Observations

Observations in science.

An observation is some thing, event, or phenomenon that is noticed or observed. Observations are listed as the first step in the scientific method because they often provide a starting point, a source of questions a researcher may ask. For example, the observation that leaves change color in the fall may lead a researcher to ask why this is so, and to propose a hypothesis to explain this phenomena. In fact, observations also will provide the key to answering the research question.

In science, observations form the foundation of all hypotheses, experiments, and theories. In an experiment, the researcher carefully plans what observations will be made and how they will be recorded. To be accepted, scientific conclusions and theories must be supported by all available observations. If new observations are made which seem to contradict an established theory, that theory will be re-examined and may be revised to explain the new facts. Observations are the nuts and bolts of science that researchers use to piece together a better understanding of nature.

Observations in science are made in a way that can be precisely communicated to (and verified by) other researchers. In many types of studies (especially in chemistry, physics, and biology), quantitative observations are used. A quantitative observation is one that is expressed and recorded as a quantity, using some standard system of measurement. Quantities such as size, volume, weight, time, distance, or a host of others may be measured in scientific studies.

Some observations that researchers need to make may be difficult or impossible to quantify. Take the example of color. Not all individuals perceive color in exactly the same way. Even apart from limiting conditions such as colorblindness, the way two people see and describe the color of a particular flower, for example, will not be the same. Color, as perceived by the human eye, is an example of a qualitative observation.

Qualitative observations note qualities associated with subjects or samples that are not readily measured. Other examples of qualitative observations might be descriptions of mating behaviors, human facial expressions, or "yes/no" type of data, where some factor is present or absent. Though the qualities of an object may be more difficult to describe or measure than any quantities associated with it, every attempt is made to minimize the effects of the subjective perceptions of the researcher in the process. Some types of studies, such as those in the social and behavioral sciences (which deal with highly variable human subjects), may rely heavily on qualitative observations.

Question: Why are observations important to science?

Limits of Observations

Because all observations rely to some degree on the senses (eyes, ears, or steady hand) of the researcher, complete objectivity is impossible. Our human perceptions are limited by the physical abilities of our sense organs and are interpreted according to our understanding of how the world works, which can be influenced by culture, experience, or education. According to science education specialist, George F. Kneller, "Surprising as it may seem, there is no fact that is not colored by our preconceptions" ("A Method of Enquiry," from Science and Its Ways of Knowing [Upper Saddle River: Prentice-Hall Inc., 1997], 15).

Observations made by a scientist are also limited by the sensitivity of whatever equipment he is using. Research findings will be limited at times by the available technology. For example, Italian physicist and philosopher Galileo Galilei (1564–1642) was reportedly the first person to observe the heavens with a telescope. Imagine how it must have felt to him to see the heavens through this amazing new instrument! It opened a window to the stars and planets and allowed new observations undreamed of before.

In the centuries since Galileo, increasingly more powerful telescopes have been devised that dwarf the power of that first device. In the past decade, we have marveled at images from deep space , courtesy of the Hubble Space Telescope, a large telescope that orbits Earth. Because of its view from outside the distorting effects of the atmosphere, the Hubble can look 50 times farther into space than the best earth-bound telescopes, and resolve details a tenth of the size (Seeds, Michael A., Horizons: Exploring the Universe , 5 th ed. [Belmont: Wadsworth Publishing Company, 1998], 86-87).

Construction is underway on a new radio telescope that scientists say will be able to detect electromagnetic waves from the very edges of the universe! This joint U.S.-Mexican project may allow us to ask questions about the origins of the universe and the beginnings of time that we could never have hoped to answer before. Completion of the new telescope is expected by the end of 2001.

Although the amount of detail observed by Galileo and today's astronomers is vastly different, the stars and their relationships have not changed very much. Yet with each technological advance, the level of detail of observation has been increased, and with it, the power to answer more and more challenging questions with greater precision.

Question: What are some of the differences between a casual observation and a 'scientific observation'?

Step 2: The Hypothesis

A hypothesis is a statement created by the researcher as a potential explanation for an observation or phenomena. The hypothesis converts the researcher's original question into a statement that can be used to make predictions about what should be observed if the hypothesis is true. For example, given the hypothesis, "exposure to ultraviolet (UV) radiation increases the risk of skin cancer," one would predict higher rates of skin cancer among people with greater UV exposure. These predictions could be tested by comparing skin cancer rates among individuals with varying amounts of UV exposure. Note how the hypothesis itself determines what experiments or further observations should be made to test its validity. Results of tests are then compared to predictions from the hypothesis, and conclusions are stated in terms of whether or not the data supports the hypothesis. So the hypothesis serves a guide to the full process of scientific inquiry.

The Qualities of a Good Hypothesis

• A hypothesis must be testable or provide predictions that are testable. It can potentially be shown to be false by further observations or experimentation.
• A hypothesis should be specific. If it is too general it cannot be tested, or tests will have so many variables that the results will be complicated and difficult to interpret. A well-written hypothesis is so specific it actually determines how the experiment should be set up.
• A hypothesis should not include any untested assumptions if they can be avoided. The hypothesis itself may be an assumption that is being tested, but it should be phrased in a way that does not include assumptions that are not tested in the experiment.
• It is okay (and sometimes a good idea) to develop more than one hypothesis to explain a set of observations. Competing hypotheses can often be tested side-by-side in the same experiment.

Question: Why is the hypothesis important to the scientific method?

Step 3: Testing the Hypothesis

A hypothesis may be tested in one of two ways: by making additional observations of a natural situation, or by setting up an experiment. In either case, the hypothesis is used to make predictions, and the observations or experimental data collected are examined to determine if they are consistent or inconsistent with those predictions. Hypothesis testing, especially through experimentation, is at the core of the scientific process. It is how scientists gain a better understanding of how things work.

Testing a Hypothesis by Observation

Some hypotheses may be tested through simple observation. For example, a researcher may formulate the hypothesis that the sun always rises in the east. What might an alternative hypothesis be? If his hypothesis is correct, he would predict that the sun will rise in the east tomorrow. He can easily test such a prediction by rising before dawn and going out to observe the sunrise. If the sun rises in the west, he will have disproved the hypothesis. He will have shown that it does not hold true in every situation. However, if he observes on that morning that the sun does in fact rise in the east, he has not proven the hypothesis. He has made a single observation that is consistent with, or supports, the hypothesis. As a scientist, to confidently state that the sun will always rise in the east, he will want to make many observations, under a variety of circumstances. Note that in this instance no manipulation of circumstance is required to test the hypothesis (i.e., you aren't altering the sun in any way).

Testing a Hypothesis by Experimentation

An experiment is a controlled series of observations designed to test a specific hypothesis. In an experiment, the researcher manipulates factors related to the hypothesis in such a way that the effect of these factors on the observations (data) can be readily measured and compared. Most experiments are an attempt to define a cause-and-effect relationship between two factors or events—to explain why something happens. For example, with the hypothesis "roses planted in sunny areas bloom earlier than those grown in shady areas," the experiment would be testing a cause-and-effect relationship between sunlight and time of blooming.

A major advantage of setting up an experiment versus making observations of what is already available is that it allows the researcher to control all the factors or events related to the hypothesis, so that the true cause of an event can be more easily isolated. In all cases, the hypothesis itself will determine the way the experiment will be set up. For example, suppose my hypothesis is "the weight of an object is proportional to the amount of time it takes to fall a certain distance." How would you test this hypothesis?

The Qualities of a Good Experiment

• The experiment must be conducted on a group of subjects that are narrowly defined and have certain aspects in common. This is the group to which any conclusions must later be confined. (Examples of possible subjects: female cancer patients over age 40, E. coli bacteria, red giant stars, the nicotine molecule and its derivatives.)
• All subjects of the experiment should be (ideally) completely alike in all ways except for the factor or factors that are being tested. Factors that are compared in scientific experiments are called variables. A variable is some aspect of a subject or event that may differ over time or from one group of subjects to another. For example, if a biologist wanted to test the effect of nitrogen on grass growth, he would apply different amounts of nitrogen fertilizer to several plots of grass. The grass in each of the plots should be as alike as possible so that any difference in growth could be attributed to the effect of the nitrogen. For example, all the grass should be of the same species, planted at the same time and at the same density, receive the same amount of water and sunlight, and so on. The variable in this case would be the amount of nitrogen applied to the plants. The researcher would not compare differing amounts of nitrogen across different grass species to determine the effect of nitrogen on grass growth. What is the problem with using different species of plants to compare the effect of nitrogen on plant growth? There are different kinds of variables in an experiment. A factor that the experimenter controls, and changes intentionally to determine if it has an effect, is called an independent variable . A factor that is recorded as data in the experiment, and which is compared across different groups of subjects, is called a dependent variable . In many cases, the value of the dependent variable will be influenced by the value of an independent variable. The goal of the experiment is to determine a cause-and-effect relationship between independent and dependent variables—in this case, an effect of nitrogen on plant growth. In the nitrogen/grass experiment, (1) which factor was the independent variable? (2) Which factor was the dependent variable?
• Nearly all types of experiments require a control group and an experimental group. The control group generally is not changed in any way, but remains in a "natural state," while the experimental group is modified in some way to examine the effect of the variable which of interest to the researcher. The control group provides a standard of comparison for the experimental groups. For example, in new drug trials, some patients are given a placebo while others are given doses of the drug being tested. The placebo serves as a control by showing the effect of no drug treatment on the patients. In research terminology, the experimental groups are often referred to as treatments , since each group is treated differently. In the experimental test of the effect of nitrogen on grass growth, what is the control group? In the example of the nitrogen experiment, what is the purpose of a control group?
• In research studies a great deal of emphasis is placed on repetition. It is essential that an experiment or study include enough subjects or enough observations for the researcher to make valid conclusions. The two main reasons why repetition is important in scientific studies are (1) variation among subjects or samples and (2) measurement error.

Variation among Subjects

There is a great deal of variation in nature. In a group of experimental subjects, much of this variation may have little to do with the variables being studied, but could still affect the outcome of the experiment in unpredicted ways. For example, in an experiment designed to test the effects of alcohol dose levels on reflex time in 18- to 22-year-old males, there would be significant variation among individual responses to various doses of alcohol. Some of this variation might be due to differences in genetic make-up, to varying levels of previous alcohol use, or any number of factors unknown to the researcher.

Because what the researcher wants to discover is average dose level effects for this group, he must run the test on a number of different subjects. Suppose he performed the test on only 10 individuals. Do you think the average response calculated would be the same as the average response of all 18- to 22-year-old males? What if he tests 100 individuals, or 1,000? Do you think the average he comes up with would be the same in each case? Chances are it would not be. So which average would you predict would be most representative of all 18- to 22-year-old males?

A basic rule of statistics is, the more observations you make, the closer the average of those observations will be to the average for the whole population you are interested in. This is because factors that vary among a population tend to occur most commonly in the middle range, and least commonly at the two extremes. Take human height for example. Although you may find a man who is 7 feet tall, or one who is 4 feet tall, most men will fall somewhere between 5 and 6 feet in height. The more men we measure to determine average male height, the less effect those uncommon extreme (tall or short) individuals will tend to impact the average. Thus, one reason why repetition is so important in experiments is that it helps to assure that the conclusions made will be valid not only for the individuals tested, but also for the greater population those individuals represent.

"The use of a sample (or subset) of a population, an event, or some other aspect of nature for an experimental group that is not large enough to be representative of the whole" is called sampling error (Starr, Cecie, Biology: Concepts and Applications , 4 th ed. [Pacific Cove: Brooks/Cole, 2000], glossary). If too few samples or subjects are used in an experiment, the researcher may draw incorrect conclusions about the population those samples or subjects represent.

Use the jellybean activity below to see a simple demonstration of samping error.

Directions: There are 400 jellybeans in the jar. If you could not see the jar and you initially chose 1 green jellybean from the jar, you might assume the jar only contains green jelly beans. The jar actually contains both green and black jellybeans. Use the "pick 1, 5, or 10" buttons to create your samples. For example, use the "pick" buttons now to create samples of 2, 13, and 27 jellybeans. After you take each sample, try to predict the ratio of green to black jellybeans in the jar. How does your prediction of the ratio of green to black jellybeans change as your sample changes?

Measurement Error

The second reason why repetition is necessary in research studies has to do with measurement error. Measurement error may be the fault of the researcher, a slight difference in measuring techniques among one or more technicians, or the result of limitations or glitches in measuring equipment. Even the most careful researcher or the best state-of-the-art equipment will make some mistakes in measuring or recording data. Another way of looking at this is to say that, in any study, some measurements will be more accurate than others will. If the researcher is conscientious and the equipment is good, the majority of measurements will be highly accurate, some will be somewhat inaccurate, and a few may be considerably inaccurate. In this case, the same reasoning used above also applies here: the more measurements taken, the less effect a few inaccurate measurements will have on the overall average.

Step 4: Data Analysis

In any experiment, observations are made, and often, measurements are taken. Measurements and observations recorded in an experiment are referred to as data . The data collected must relate to the hypothesis being tested. Any differences between experimental and control groups must be expressed in some way (often quantitatively) so that the groups may be compared. Graphs and charts are often used to visualize the data and to identify patterns and relationships among the variables.

Statistics is the branch of mathematics that deals with interpretation of data. Data analysis refers to statistical methods of determining whether any differences between the control group and experimental groups are too great to be attributed to chance alone. Although a discussion of statistical methods is beyond the scope of this tutorial, the data analysis step is crucial because it provides a somewhat standardized means for interpreting data. The statistical methods of data analysis used, and the results of those analyses, are always included in the publication of scientific research. This convention limits the subjective aspects of data interpretation and allows scientists to scrutinize the working methods of their peers.

Why is data analysis an important step in the scientific method?

Step 5: Stating Conclusions

The conclusions made in a scientific experiment are particularly important. Often, the conclusion is the only part of a study that gets communicated to the general public. As such, it must be a statement of reality, based upon the results of the experiment. To assure that this is the case, the conclusions made in an experiment must (1) relate back to the hypothesis being tested, (2) be limited to the population under study, and (3) be stated as probabilities.

The hypothesis that is being tested will be compared to the data collected in the experiment. If the experimental results contradict the hypothesis, it is rejected and further testing of that hypothesis under those conditions is not necessary. However, if the hypothesis is not shown to be wrong, that does not conclusively prove that it is right! In scientific terms, the hypothesis is said to be "supported by the data." Further testing will be done to see if the hypothesis is supported under a number of trials and under different conditions.

If the hypothesis holds up to extensive testing then the temptation is to claim that it is correct. However, keep in mind that the number of experiments and observations made will only represent a subset of all the situations in which the hypothesis may potentially be tested. In other words, experimental data will only show part of the picture. There is always the possibility that a further experiment may show the hypothesis to be wrong in some situations. Also, note that the limits of current knowledge and available technologies may prevent a researcher from devising an experiment that would disprove a particular hypothesis.

The researcher must be sure to limit his or her conclusions to apply only to the subjects tested in the study. If a particular species of fish is shown to consume their young 90 percent of the time when raised in captivity, that doesn't necessarily mean that all fish will do so, or that this fish's behavior would be the same in its native habitat.

Finally, the conclusions of the experiment are generally stated as probabilities. A careful scientist would never say, "drug x kills cancer cells;" she would more likely say, "drug x was shown to destroy 85 percent of cancerous skin cells in rats in lab trials." Notice how very different these two statements are. There is a tendency in the media and in the general public to gravitate toward the first statement. This makes a terrific headline and is also easy to interpret; it is absolute. Remember though, in science conclusions must be confined to the population under study; broad generalizations should be avoided. The second statement is sound science. There is data to back it up. Later studies may reveal a more universal effect of the drug on cancerous cells, or they may not. Most researchers would be unwilling to stake their reputations on the first statement.

As a student, you should read and interpret popular press articles about research studies very carefully. From the text, can you determine how the experiment was set up and what variables were measured? Are the observations and data collected appropriate to the hypothesis being tested? Are the conclusions supported by the data? Are the conclusions worded in a scientific context (as probability statements) or are they generalized for dramatic effect? In any researched-based assignment, it is a good idea to refer to the original publication of a study (usually found in professional journals) and to interpret the facts for yourself.

Qualities of a Good Experiment

• narrowly defined subjects
• all subjects treated alike except for the factor or variable being studied
• a control group is used for comparison
• measurements related to the factors being studied are carefully recorded
• enough samples or subjects are used so that conclusions are valid for the population of interest
• conclusions made relate back to the hypothesis, are limited to the population being studied, and are stated in terms of probabilities

The Scientific Method by Science Made Simple

Understanding and using the scientific method.

The Scientific Method is a process used to design and perform experiments. It's important to minimize experimental errors and bias, and increase confidence in the accuracy of your results.

In the previous sections, we talked about how to pick a good topic and specific question to investigate. Now we will discuss how to carry out your investigation.

Steps of the Scientific Method

• Observation/Research
• Experimentation

Now that you have settled on the question you want to ask, it's time to use the Scientific Method to design an experiment to answer that question.

If your experiment isn't designed well, you may not get the correct answer. You may not even get any definitive answer at all!

The Scientific Method is a logical and rational order of steps by which scientists come to conclusions about the world around them. The Scientific Method helps to organize thoughts and procedures so that scientists can be confident in the answers they find.

OBSERVATION is first step, so that you know how you want to go about your research.

HYPOTHESIS is the answer you think you'll find.

PREDICTION is your specific belief about the scientific idea: If my hypothesis is true, then I predict we will discover this.

EXPERIMENT is the tool that you invent to answer the question, and

CONCLUSION is the answer that the experiment gives.

Don't worry, it isn't that complicated. Let's take a closer look at each one of these steps. Then you can understand the tools scientists use for their science experiments, and use them for your own.

OBSERVATION

This step could also be called "research." It is the first stage in understanding the problem.

After you decide on topic, and narrow it down to a specific question, you will need to research everything that you can find about it. You can collect information from your own experiences, books, the internet, or even smaller "unofficial" experiments.

Let's continue the example of a science fair idea about tomatoes in the garden. You like to garden, and notice that some tomatoes are bigger than others and wonder why.

Because of this personal experience and an interest in the problem, you decide to learn more about what makes plants grow.

For this stage of the Scientific Method, it's important to use as many sources as you can find. The more information you have on your science fair topic, the better the design of your experiment is going to be, and the better your science fair project is going to be overall.

Also try to get information from your teachers or librarians, or professionals who know something about your science fair project. They can help to guide you to a solid experimental setup.

The next stage of the Scientific Method is known as the "hypothesis." This word basically means "a possible solution to a problem, based on knowledge and research."

The hypothesis is a simple statement that defines what you think the outcome of your experiment will be.

All of the first stage of the Scientific Method -- the observation, or research stage -- is designed to help you express a problem in a single question ("Does the amount of sunlight in a garden affect tomato size?") and propose an answer to the question based on what you know. The experiment that you will design is done to test the hypothesis.

Using the example of the tomato experiment, here is an example of a hypothesis:

TOPIC: "Does the amount of sunlight a tomato plant receives affect the size of the tomatoes?"

HYPOTHESIS: "I believe that the more sunlight a tomato plant receives, the larger the tomatoes will grow.

This hypothesis is based on:

(1) Tomato plants need sunshine to make food through photosynthesis, and logically, more sun means more food, and;

(2) Through informal, exploratory observations of plants in a garden, those with more sunlight appear to grow bigger.

The hypothesis is your general statement of how you think the scientific phenomenon in question works.

Your prediction lets you get specific -- how will you demonstrate that your hypothesis is true? The experiment that you will design is done to test the prediction.

An important thing to remember during this stage of the scientific method is that once you develop a hypothesis and a prediction, you shouldn't change it, even if the results of your experiment show that you were wrong.

An incorrect prediction does NOT mean that you "failed." It just means that the experiment brought some new facts to light that maybe you hadn't thought about before.

Continuing our tomato plant example, a good prediction would be: Increasing the amount of sunlight tomato plants in my experiment receive will cause an increase in their size compared to identical plants that received the same care but less light.

This is the part of the scientific method that tests your hypothesis. An experiment is a tool that you design to find out if your ideas about your topic are right or wrong.

It is absolutely necessary to design a science fair experiment that will accurately test your hypothesis. The experiment is the most important part of the scientific method. It's the logical process that lets scientists learn about the world.

On the next page, we'll discuss the ways that you can go about designing a science fair experiment idea.

The final step in the scientific method is the conclusion. This is a summary of the experiment's results, and how those results match up to your hypothesis.

You have two options for your conclusions: based on your results, either:

(1) YOU CAN REJECT the hypothesis, or

(2) YOU CAN NOT REJECT the hypothesis.

This is an important point!

You can not PROVE the hypothesis with a single experiment, because there is a chance that you made an error somewhere along the way.

What you can say is that your results SUPPORT the original hypothesis.

If your original hypothesis didn't match up with the final results of your experiment, don't change the hypothesis.

Instead, try to explain what might have been wrong with your original hypothesis. What information were you missing when you made your prediction? What are the possible reasons the hypothesis and experimental results didn't match up?

Remember, a science fair experiment isn't a failure simply because does not agree with your hypothesis. No one will take points off if your prediction wasn't accurate. Many important scientific discoveries were made as a result of experiments gone wrong!

A science fair experiment is only a failure if its design is flawed. A flawed experiment is one that (1) doesn't keep its variables under control, and (2) doesn't sufficiently answer the question that you asked of it.

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1.3: The Scientific Method

Chapter 1: scientific inquiry, chapter 2: chemistry of life, chapter 3: macromolecules, chapter 4: cell structure and function, chapter 5: membranes and cellular transport, chapter 6: cell signaling, chapter 7: metabolism, chapter 8: cellular respiration, chapter 9: photosynthesis, chapter 10: cell cycle and division, chapter 11: meiosis, chapter 12: classical and modern genetics, chapter 13: dna structure and function, chapter 14: gene expression, chapter 15: biotechnology, chapter 16: viruses, chapter 17: nutrition and digestion, chapter 18: nervous system, chapter 19: sensory systems, chapter 20: musculoskeletal system, chapter 21: endocrine system, chapter 22: circulatory and pulmonary systems, chapter 23: osmoregulation and excretion, chapter 24: immune system, chapter 25: reproduction and development, chapter 26: behavior, chapter 27: ecosystems, chapter 28: population and community ecology, chapter 29: biodiversity and conservation, chapter 30: speciation and diversity, chapter 31: natural selection, chapter 32: population genetics, chapter 33: evolutionary history, chapter 34: plant structure, growth, and nutrition, chapter 35: plant reproduction, chapter 36: plant responses to the environment.

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The scientific method is a detailed, stepwise process for answering questions. For example, a scientist makes an observation that the slugs destroy some cabbages but not those near garlic.

Such observations lead to asking questions, "Could garlic be used to deter slugs from ruining a cabbage patch?" After formulating questions, the scientist can then develop hypotheses —potential explanations for the observations that lead to specific, testable predictions.

In this case, a hypothesis could be that garlic repels slugs, which predicts that cabbages surrounded by garlic powder will suffer less damage than the ones without it. 

The hypothesis is then tested through a series of experiments designed to eliminate hypotheses.

The experimental setup involves defining variables. An independent variable is an item that is being tested, in this case, garlic addition. The dependent variable describes the measurement used to determine the outcome, such as the number of slugs on the cabbages.

In addition, the slugs must be divided into groups, experimental and control. These groups are identical, except that the experimental group is exposed to garlic powder.

After data are collected and analyzed, conclusions are made, and results are communicated to other scientists.

The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves formulating a question based on observation, developing a testable potential explanation for the observation (called a hypothesis), making and testing predictions based on the hypothesis, and using the findings to create new hypotheses and predictions.

Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these experiments, the original hypothesis may need to be refined, and new hypotheses and questions can be generated. Importantly, this illustrates that the scientific method is not a stepwise recipe. Instead, it is a continuous refinement and testing of ideas based on new observations, which is the crux of scientific inquiry.

Science is mutable and continuously changes as scientists learn more about the world, physical phenomena and how organisms interact with their environment. For this reason, scientists avoid claiming to ‘prove' a specific idea. Instead, they gather evidence that either supports or refutes a given hypothesis.

Making Observations and Formulating Hypotheses

A hypothesis is preceded by an initial observation, during which information is gathered by the senses (e.g., vision, hearing) or using scientific tools and instruments. This observation leads to a question that prompts the formation of an initial hypothesis, a (testable) possible answer to the question. For example, the observation that slugs eat some cabbage plants but not cabbage plants located near garlic may prompt the question: why do slugs selectively not eat cabbage plants near garlic? One possible hypothesis, or answer to this question, is that slugs have an aversion to garlic. Based on this hypothesis, one might predict that slugs will not eat cabbage plants surrounded by a ring of garlic powder.

A hypothesis should be falsifiable, meaning that there are ways to disprove it if it is untrue. In other words, a hypothesis should be testable. Scientists often articulate and explicitly test for the opposite of the hypothesis, which is called the null hypothesis. In this case, the null hypothesis is that slugs do not have an aversion to garlic. The null hypothesis would be supported if, contrary to the prediction, slugs eat cabbage plants that are surrounded by garlic powder.

Testing a Hypothesis

When possible, scientists test hypotheses using controlled experiments that include independent and dependent variables, as well as control and experimental groups.

An independent variable is an item expected to have an effect (e.g., the garlic powder used in the slug and cabbage experiment or treatment given in a clinical trial). Dependent variables are the measurements used to determine the outcome of an experiment. In the experiment with slugs, cabbages, and garlic, the number of slugs eating cabbages is the dependent variable. This number is expected to depend on the presence or absence of garlic powder rings around the cabbage plants.

Experiments require experimental and control groups. An experimental group is treated with or exposed to the independent variable (i.e., the manipulation or treatment). For example, in the garlic aversion experiment with slugs, the experimental group is a group of cabbage plants surrounded by a garlic powder ring. A control group is subject to the same conditions as the experimental group, with the exception of the independent variable. Control groups in this experiment might include a group of cabbage plants in the same area that is surrounded by a non-garlic powder ring (to control for powder aversion) and a group that is not surrounded by any particular substance (to control for cabbage aversion). It is essential to include a control group because, without one, it is unclear whether the outcome is the result of the treatment or manipulation.

Refining a Hypothesis

If the results of an experiment support the hypothesis, further experiments may be designed and carried out to provide support for the hypothesis. The hypothesis may also be refined and made more specific. For example, additional experiments could determine whether slugs also have an aversion to other plants of the Allium genus, like onions.

If the results do not support the hypothesis, then the original hypothesis may be modified based on the new observations. It is important to rule out potential problems with the experimental design before modifying the hypothesis. For example, if slugs demonstrate an aversion to both garlic and non-garlic powder, the experiment can be carried out again using fresh garlic instead of powdered garlic. If the slugs still exhibit no aversion to garlic, then the original hypothesis can be modified.

Communication

The results of the experiments should be communicated to other scientists and the public, regardless of whether the data support the original hypothesis. This information can guide the development of new hypotheses and experimental questions.

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Scientific Method: Step 6: CONCLUSION

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 6: Conclusion

Finally, you've reached your conclusion. Now it is time to summarize and explain what happened in your experiment. Your conclusion should answer the question posed in step one. Your conclusion should be based solely on your results.

Think about the following questions:

• Was your hypothesis correct?
• If your hypothesis wasn't correct, what can you conclude from that?
• Do you need to run your experiment again changing a variable?
• Is your data clearly defined so everyone can understand the results and follow your reasoning?

Remember, even a failed experiment can yield a valuable lesson.  

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1.1: Scientific Investigation

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Chances are you've heard of the scientific method. But what exactly is the scientific method?

Is it a precise and exact way that all science must be done? Or is it a series of steps that most scientists generally follow, but may be modified for the benefit of an individual investigation?

The Scientific Method

"We also discovered that science is cool and fun because you get to do stuff that no one has ever done before." In the article Blackawton bees, published by eight to ten year old students: Biology Letters (2010) http://rsbl.royalsocietypublishing.org/content/early/2010/12/18/rsbl.2010.1056.abstract .

There are basic methods of gaining knowledge that are common to all of science. At the heart of science is the scientific investigation, which is done by following the scientific method . A scientific investigation is a plan for asking questions and testing possible answers. It generally follows the steps listed in Figure below . See http://www.youtube.com/watch?v=KZaCy5Z87FA for an overview of the scientific method.

Steps of a Scientific Investigation. A scientific investigation typically has these steps. Scientists often develop their own steps they follow in a scientific investigation. Shown here is a simplification of how a scientific investigation is done.

Making Observations

A scientific investigation typically begins with observations. You make observations all the time. Let’s say you take a walk in the woods and observe a moth, like the one in Figure below, resting on a tree trunk. You notice that the moth has spots on its wings that look like eyes. You think the eye spots make the moth look like the face of an owl.

Figure 2: Marbled emperor moth Heniocha dyops in Botswana. (CC-SA-BY-4.0; Charlesjsharp ).

Does this moth remind you of an owl?

Asking a Question

Observations often lead to questions. For example, you might ask yourself why the moth has eye spots that make it look like an owl’s face. What reason might there be for this observation?

Forming a Hypothesis

The next step in a scientific investigation is forming a hypothesis . A hypothesis is a possible answer to a scientific question, but it isn’t just any answer. A hypothesis must be based on scientific knowledge, and it must be logical. A hypothesis also must be falsifiable. In other words, it must be possible to make observations that would disprove the hypothesis if it really is false. Assume you know that some birds eat moths and that owls prey on other birds. From this knowledge, you reason that eye spots scare away birds that might eat the moth. This is your hypothesis.

Testing the Hypothesis

To test a hypothesis, you first need to make a prediction based on the hypothesis. A prediction is a statement that tells what will happen under certain conditions. It can be expressed in the form: If A occurs, then B will happen. Based on your hypothesis, you might make this prediction: If a moth has eye spots on its wings, then birds will avoid eating it.

Next, you must gather evidence to test your prediction. Evidence is any type of data that may either agree or disagree with a prediction, so it may either support or disprove a hypothesis. Evidence may be gathered by an experiment . Assume that you gather evidence by making more observations of moths with eye spots. Perhaps you observe that birds really do avoid eating moths with eye spots. This evidence agrees with your prediction.

Drawing Conclusions

Evidence that agrees with your prediction supports your hypothesis. Does such evidence prove that your hypothesis is true? No; a hypothesis cannot be proven conclusively to be true. This is because you can never examine all of the possible evidence, and someday evidence might be found that disproves the hypothesis. Nonetheless, the more evidence that supports a hypothesis, the more likely the hypothesis is to be true.

Communicating Results

The last step in a scientific investigation is communicating what you have learned with others. This is a very important step because it allows others to test your hypothesis. If other researchers get the same results as yours, they add support to the hypothesis. However, if they get different results, they may disprove the hypothesis.

When scientists share their results, they should describe their methods and point out any possible problems with the investigation. For example, while you were observing moths, perhaps your presence scared birds away. This introduces an error into your investigation. You got the results you predicted (the birds avoided the moths while you were observing them), but not for the reason you hypothesized. Other researchers might be able to think of ways to avoid this error in future studies.

The Scientific Method Made Easy explains scientific method: http://www.youtube.com/watch?v=zcavPAFiG14 (9:55).

As you view The Scientific Method Made Easy, focus on these concepts: the relationship between evidence, conclusions and theories, the "ground rules" of scientific research, the steps in a scientific procedure, the meaning of the "replication of results," the meaning of "falsifiable," the outcome when the scientific method is not followed.

Discovering the Scientific Method

A summery video of the scientific method, using the identification of DNA structure as an example, is shown in this video by MIT students: https://www.youtube.com/watch?v=5eDNgeEUtMg .

Why I do Science

Dan Costa, Ph.D. is a professor of Biology at the University of California, Santa Cruz, and has been studying marine life for well over 40 years. He is a leader in using satellite tags, time and depth recorders and other sophisticated electronic tags to gather information about the amazing depths to which elephant seals dive, their migration routes and how they use oceanographic features to hunt for prey as far as the international dateline and the Alaskan Aleutian Islands. In the following KQED video, Dr. Costa discusses why he is a scientist: http://science.kqed.org/quest/video/why-i-do-science-dan-costa/ .

• At the heart of science is the scientific investigation, which is done by following the scientific method. A scientific investigation is a plan for asking questions and testing possible answers.
• A scientific investigation typically begins with observations. Observations often lead to questions.
• A hypothesis is a possible logical answer to a scientific question, based on scientific knowledge.
• A prediction is a statement that tells what will happen under certain conditions.
• Evidence is any type of data that may either agree or disagree with a prediction, so it may either support or disprove a hypothesis. Conclusions may be formed from evidence.
• The last step in a scientific investigation is the communication of results with others.

Explore More

Explore more i.

Use this resource to answer the questions that follow.

• Steps of the Scientific Method at http://www.sciencebuddies.org/science-fair-projects/project_scientific_method.shtml#overviewofthescientificmethod .
• Describe what is means to "Ask a Question."
• Describe what it means to "Construct a Hypothesis."
• How does a scientist conduct a fair test?
• What does a scientist do if the hypothesis is not supported?

Explore More II

• SciMeth Matching at http://www.studystack.com/matching-2497 .
• Outline the steps of a scientific investigation.
• What is a scientific hypothesis? What characteristics must a hypothesis have to be useful in science?
• Give an example of a scientific question that could be investigated with an experiment. Then give an example of question that could not be investigated.
• Can a hypothesis be proven true? Why or why not?
• Why do scientists communicate their results?

Six Steps of the Scientific Method

Learn What Makes Each Stage Important

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The scientific method is a systematic way of learning about the world around us and answering questions. The key difference between the scientific method and other ways of acquiring knowledge are forming a hypothesis and then testing it with an experiment.

The Six Steps

The number of steps can vary from one description to another (which mainly happens when data and analysis are separated into separate steps), however, this is a fairly standard list of the six scientific method steps that you are expected to know for any science class:

• Purpose/Question Ask a question.
• Research Conduct background research. Write down your sources so you can cite your references. In the modern era, a lot of your research may be conducted online. Scroll to the bottom of articles to check the references. Even if you can't access the full text of a published article, you can usually view the abstract to see the summary of other experiments. Interview experts on a topic. The more you know about a subject, the easier it will be to conduct your investigation.
• Hypothesis Propose a hypothesis . This is a sort of educated guess about what you expect. It is a statement used to predict the outcome of an experiment. Usually, a hypothesis is written in terms of cause and effect. Alternatively, it may describe the relationship between two phenomena. One type of hypothesis is the null hypothesis or the no-difference hypothesis. This is an easy type of hypothesis to test because it assumes changing a variable will have no effect on the outcome. In reality, you probably expect a change but rejecting a hypothesis may be more useful than accepting one.
• Experiment Design and perform an experiment to test your hypothesis. An experiment has an independent and dependent variable. You change or control the independent variable and record the effect it has on the dependent variable . It's important to change only one variable for an experiment rather than try to combine the effects of variables in an experiment. For example, if you want to test the effects of light intensity and fertilizer concentration on the growth rate of a plant, you're really looking at two separate experiments.
• Data/Analysis Record observations and analyze the meaning of the data. Often, you'll prepare a table or graph of the data. Don't throw out data points you think are bad or that don't support your predictions. Some of the most incredible discoveries in science were made because the data looked wrong! Once you have the data, you may need to perform a mathematical analysis to support or refute your hypothesis.
• Conclusion Conclude whether to accept or reject your hypothesis. There is no right or wrong outcome to an experiment, so either result is fine. Accepting a hypothesis does not necessarily mean it's correct! Sometimes repeating an experiment may give a different result. In other cases, a hypothesis may predict an outcome, yet you might draw an incorrect conclusion. Communicate your results. The results may be compiled into a lab report or formally submitted as a paper. Whether you accept or reject the hypothesis, you likely learned something about the subject and may wish to revise the original hypothesis or form a new one for a future experiment.

When Are There Seven Steps?

Sometimes the scientific method is taught with seven steps instead of six. In this model, the first step of the scientific method is to make observations. Really, even if you don't make observations formally, you think about prior experiences with a subject in order to ask a question or solve a problem.

Formal observations are a type of brainstorming that can help you find an idea and form a hypothesis. Observe your subject and record everything about it. Include colors, timing, sounds, temperatures, changes, behavior, and anything that strikes you as interesting or significant.

When you design an experiment, you are controlling and measuring variables. There are three types of variables:

• Controlled Variables:  You can have as many  controlled variables  as you like. These are parts of the experiment that you try to keep constant throughout an experiment so that they won't interfere with your test. Writing down controlled variables is a good idea because it helps make your experiment  reproducible , which is important in science! If you have trouble duplicating results from one experiment to another, there may be a controlled variable that you missed.
• Independent Variable:  This is the variable you control.
• Dependent Variable:  This is the variable you measure. It is called the dependent variable because it  depends  on the independent variable.
• Scientific Method Flow Chart
• What Is an Experiment? Definition and Design
• How To Design a Science Fair Experiment
• What Is a Hypothesis? (Science)
• Scientific Variable
• What Are the Elements of a Good Hypothesis?
• Scientific Method Vocabulary Terms
• Understanding Simple vs Controlled Experiments
• Examples of Independent and Dependent Variables
• What Is a Variable in Science?
• Null Hypothesis Examples
• Null Hypothesis Definition and Examples
• Independent Variable Definition and Examples
• Scientific Method Lesson Plan
• Dependent Variable Definition and Examples

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High school biology

Course: high school biology   >   unit 1.

• Biology overview
• Preparing to study biology
• What is life?
• The scientific method
• Data to justify experimental claims examples
• Scientific method and data analysis
• Introduction to experimental design
• Controlled experiments

Biology and the scientific method review

• Experimental design and bias

The nature of biology

Properties of life.

• Organization: Living things are highly organized (meaning they contain specialized, coordinated parts) and are made up of one or more cells .
• Metabolism: Living things must use energy and consume nutrients to carry out the chemical reactions that sustain life. The sum total of the biochemical reactions occurring in an organism is called its metabolism .
• Homeostasis : Living organisms regulate their internal environment to maintain the relatively narrow range of conditions needed for cell function.
• Growth : Living organisms undergo regulated growth. Individual cells become larger in size, and multicellular organisms accumulate many cells through cell division.
• Reproduction : Living organisms can reproduce themselves to create new organisms.
• Response : Living organisms respond to stimuli or changes in their environment.
• Evolution : Populations of living organisms can undergo evolution , meaning that the genetic makeup of a population may change over time.

Scientific methodology

Scientific method example: failure to toast.

• Observation: the toaster won't toast.
• Question: Why won't my toaster toast?
• Hypothesis: Maybe the outlet is broken.
• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.
• Test of prediction: Plug the toaster into a different outlet and try again.
• Iteration time!

Experimental design

Reducing errors and bias.

• Having a large sample size in the experiment: This helps to account for any small differences among the test subjects that may provide unexpected results.
• Repeating experimental trials multiple times: Errors may result from slight differences in test subjects, or mistakes in methodology or data collection. Repeating trials helps reduce those effects.
• Including all data points: Sometimes it is tempting to throw away data points that are inconsistent with the proposed hypothesis. However, this makes for an inaccurate study! All data points need to be included, whether they support the hypothesis or not.
• Using placebos , when appropriate: Placebos prevent the test subjects from knowing whether they received a real therapeutic substance. This helps researchers determine whether a substance has a true effect.
• Implementing double-blind studies , when appropriate: Double-blind studies prevent researchers from knowing the status of a particular participant. This helps eliminate observer bias.

Communicating findings

Things to remember.

• A hypothesis is not necessarily the right explanation. Instead, it is a possible explanation that can be tested to see if it is likely correct, or if a new hypothesis needs to be made.
• Not all explanations can be considered a hypothesis. A hypothesis must be testable and falsifiable in order to be valid. For example, “The universe is beautiful" is not a good hypothesis, because there is no experiment that could test this statement and show it to be false.
• In most cases, the scientific method is an iterative process. In other words, it's a cycle rather than a straight line. The result of one experiment often becomes feedback that raises questions for more experimentation.
• Scientists use the word "theory" in a very different way than non-scientists. When many people say "I have a theory," they really mean "I have a guess." Scientific theories, on the other hand, are well-tested and highly reliable scientific explanations of natural phenomena. They unify many repeated observations and data collected from lots of experiments.

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)
• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

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• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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• How to Write Discussions and Conclusions

The discussion section contains the results and outcomes of a study. An effective discussion informs readers what can be learned from your experiment and provides context for the results.

What makes an effective discussion?

When you’re ready to write your discussion, you’ve already introduced the purpose of your study and provided an in-depth description of the methodology. The discussion informs readers about the larger implications of your study based on the results. Highlighting these implications while not overstating the findings can be challenging, especially when you’re submitting to a journal that selects articles based on novelty or potential impact. Regardless of what journal you are submitting to, the discussion section always serves the same purpose: concluding what your study results actually mean.

A successful discussion section puts your findings in context. It should include:

• the results of your research,
• a discussion of related research, and
• a comparison between your results and initial hypothesis.

Tip: Not all journals share the same naming conventions.

You can apply the advice in this article to the conclusion, results or discussion sections of your manuscript.

Our Early Career Researcher community tells us that the conclusion is often considered the most difficult aspect of a manuscript to write. To help, this guide provides questions to ask yourself, a basic structure to model your discussion off of and examples from published manuscripts. 

Questions to ask yourself:

• Was my hypothesis correct?
• If my hypothesis is partially correct or entirely different, what can be learned from the results? 
• How do the conclusions reshape or add onto the existing knowledge in the field? What does previous research say about the topic? 
• Why are the results important or relevant to your audience? Do they add further evidence to a scientific consensus or disprove prior studies? 
• How can future research build on these observations? What are the key experiments that must be done? 
• What is the “take-home” message you want your reader to leave with?

How to structure a discussion

Trying to fit a complete discussion into a single paragraph can add unnecessary stress to the writing process. If possible, you’ll want to give yourself two or three paragraphs to give the reader a comprehensive understanding of your study as a whole. Here’s one way to structure an effective discussion:

Writing Tips

While the above sections can help you brainstorm and structure your discussion, there are many common mistakes that writers revert to when having difficulties with their paper. Writing a discussion can be a delicate balance between summarizing your results, providing proper context for your research and avoiding introducing new information. Remember that your paper should be both confident and honest about the results! 

• Read the journal’s guidelines on the discussion and conclusion sections. If possible, learn about the guidelines before writing the discussion to ensure you’re writing to meet their expectations. 
• Begin with a clear statement of the principal findings. This will reinforce the main take-away for the reader and set up the rest of the discussion. 
• Explain why the outcomes of your study are important to the reader. Discuss the implications of your findings realistically based on previous literature, highlighting both the strengths and limitations of the research. 
• State whether the results prove or disprove your hypothesis. If your hypothesis was disproved, what might be the reasons? 
• Introduce new or expanded ways to think about the research question. Indicate what next steps can be taken to further pursue any unresolved questions. 
• If dealing with a contemporary or ongoing problem, such as climate change, discuss possible consequences if the problem is avoided. 
• Be concise. Adding unnecessary detail can distract from the main findings. 

Don’t

• Rewrite your abstract. Statements with “we investigated” or “we studied” generally do not belong in the discussion. 
• Include new arguments or evidence not previously discussed. Necessary information and evidence should be introduced in the main body of the paper. 
• Apologize. Even if your research contains significant limitations, don’t undermine your authority by including statements that doubt your methodology or execution. 
• Shy away from speaking on limitations or negative results. Including limitations and negative results will give readers a complete understanding of the presented research. Potential limitations include sources of potential bias, threats to internal or external validity, barriers to implementing an intervention and other issues inherent to the study design. 
• Overstate the importance of your findings. Making grand statements about how a study will fully resolve large questions can lead readers to doubt the success of the research. 

Snippets of Effective Discussions:

Consumer-based actions to reduce plastic pollution in rivers: A multi-criteria decision analysis approach

Identifying reliable indicators of fitness in polar bears

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Statistics Made Easy

How to Write Hypothesis Test Conclusions (With Examples)

A   hypothesis test is used to test whether or not some hypothesis about a population parameter is true.

To perform a hypothesis test in the real world, researchers obtain a random sample from the population and perform a hypothesis test on the sample data, using a null and alternative hypothesis:

• Null Hypothesis (H 0 ): The sample data occurs purely from chance.
• Alternative Hypothesis (H A ): The sample data is influenced by some non-random cause.

If the p-value of the hypothesis test is less than some significance level (e.g. α = .05), then we reject the null hypothesis .

Otherwise, if the p-value is not less than some significance level then we fail to reject the null hypothesis .

When writing the conclusion of a hypothesis test, we typically include:

• Whether we reject or fail to reject the null hypothesis.
• The significance level.
• A short explanation in the context of the hypothesis test.

For example, we would write:

We reject the null hypothesis at the 5% significance level.   There is sufficient evidence to support the claim that…

Or, we would write:

We fail to reject the null hypothesis at the 5% significance level.   There is not sufficient evidence to support the claim that…

The following examples show how to write a hypothesis test conclusion in both scenarios.

Example 1: Reject the Null Hypothesis Conclusion

Suppose a biologist believes that a certain fertilizer will cause plants to grow more during a one-month period than they normally do, which is currently 20 inches. To test this, she applies the fertilizer to each of the plants in her laboratory for one month.

She then performs a hypothesis test at a 5% significance level using the following hypotheses:

• H 0 : μ = 20 inches (the fertilizer will have no effect on the mean plant growth)
• H A : μ > 20 inches (the fertilizer will cause mean plant growth to increase)

Suppose the p-value of the test turns out to be 0.002.

Here is how she would report the results of the hypothesis test:

We reject the null hypothesis at the 5% significance level.   There is sufficient evidence to support the claim that this particular fertilizer causes plants to grow more during a one-month period than they normally do.

Example 2: Fail to Reject the Null Hypothesis Conclusion

Suppose the manager of a manufacturing plant wants to test whether or not some new method changes the number of defective widgets produced per month, which is currently 250. To test this, he measures the mean number of defective widgets produced before and after using the new method for one month.

He performs a hypothesis test at a 10% significance level using the following hypotheses:

• H 0 : μ after = μ before (the mean number of defective widgets is the same before and after using the new method)
• H A : μ after ≠ μ before (the mean number of defective widgets produced is different before and after using the new method)

Suppose the p-value of the test turns out to be 0.27.

Here is how he would report the results of the hypothesis test:

We fail to reject the null hypothesis at the 10% significance level.   There is not sufficient evidence to support the claim that the new method leads to a change in the number of defective widgets produced per month.

Additional Resources

The following tutorials provide additional information about hypothesis testing:

Introduction to Hypothesis Testing 4 Examples of Hypothesis Testing in Real Life How to Write a Null Hypothesis

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Hey there. My name is Zach Bobbitt. I have a Masters of Science degree in Applied Statistics and I’ve worked on machine learning algorithms for professional businesses in both healthcare and retail. I’m passionate about statistics, machine learning, and data visualization and I created Statology to be a resource for both students and teachers alike.  My goal with this site is to help you learn statistics through using simple terms, plenty of real-world examples, and helpful illustrations.

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